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ORIGINAL ARTICLE
Korean J Pediatr 2001 October;44(10) :1168-1175.
Serum Level of G-CSF and GM-CSF and CFU-GM Response to Hematopoietic Growth Factors in a Patient with Cyclic Neutropenia
Youn-Kyung Lee (Lee YK)1, Seok-Hun Kim (Kim SH)1, Kwang-Woo Kim (Kim KW)1, Soon Lee (Lee S)2, Heung-Sik Kim (Kim HS)3, Dong-Seok Chun (Chun DS)4, Kun-Soo Lee (Lee KS)5
1Department of Pediatrics, Sunlin Hospital, Handong University, Pohang, Korea
2Department of Pediatrics, Clinical Pathology , College of Medicine, Keimyung University, Korea
3Department of Pediatrics, College of Medicine, Keimyung University, Taegu, Korea
4Department of Clinical Pathology, College of Medicine, Keimyung University, Taegu, Korea
5Department of Pediatrics, College of Medicine, Kyungpook National University, Taegu, Korea
Copyright © 2001 by The Korean Pediatric Society
ABSTRACT
Purpose : Serum levels of G-CSF and GM-CSF were measured and CFU-GM assay using G- CSF, GM-CSF and SCF was conducted to evaluate the influence of hematopoietic growth factor on the precursor cells of cyclic neutropenia. Methods : A 7-year-old male with cyclic neutropenia was studied. Marrow mononuclear cells were isolated at neutrophil nadir and recovery and cultured in methylcellulose media with or without G-CSF, GM- CSF and SCF. CD34 positive cells were evaluated using flow cytometry. Serum levels of G-CSF and GM-CSF were measured by ELISA. Results : The Numbers of CFU-GM without growth factors were 50 at neutrophil nadir and 33 at the recovery phase in the patient and show increased colony forming capacity. CD34 positive cells were 9.32% at nadir and 14.17% at recovery. Increasement of CFU-GM with G-CSF at nadir and recovery were 46% and 118% and those with GM-CSF were 70% and 78% respectively, compared with 54.4% and 78.2% in control groups. In contrast, the presence of SCF did not enhance CFU-GM number in the patient, but in the control group, increasement with SCF was 28.9 %. There an was inverse relationship between serum G-CSF levels and peripheral neutrophil count whereas those of GM-CSF were constant. Conclusion : Serum G-CSF level showed inverse relationship with neutrophil counts. The response of progenitor cells to G-CSF and GM-CSF was not impaired. The presence of SCF did not enhance CFU-GM number in the patient. This result suggests that the abnormality in hematopoiesis in cyclic neutropenia may involve more immature progenitor cells responsive to SCF.
Keywords: Cyclic neutropenia | G-CSF | GM-CSF | SCF | CFU-GM | CD34
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