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ORIGINAL ARTICLE
Korean J Pediatr 2005 February;48(2) :197-203.
Gene Expression of Metalloproteinases, Tissue Inhibitors of Metalloproteinases and Cytokines in Adriamycin-induced Cardiomyopathy
Young Mi Hong (Hong YM)
Department of Pediatrics, College of Medicine, Ewha Womans University, Seoul, Korea
Corresponding Author: Young Mi Hong ,Email: hongym@chollian.net
Copyright © 2005 by The Korean Pediatric Society
ABSTRACT
ity has been reported to occur in both clinical and experimental forms of dilated cardiomyopathy. There was no report about MMP in adriamycin(ADR)-induced cardiomyopathy. The purpose of this study was to investigate gene expression of MMP and tissue inhibitor of metalloproteinases(TIMP) in ADR-induced cardiomyopathy and clarify the relationship between MMP and cytokines. Methods : Male Sprague-Dawley rats were divided into two groups. The first group was control. The second group was given intraperitoneal injections of ADR(5 mg/kg) twice a week over two weeks. Serum concentrations of MMP, TIMP, interleukin(IL)-6 and tumor necrosis factor(TNF)- were measured. RNA extraction was performed from frozen rat hearts. Reverse transcription polymerase chain reaction(RT-PCR) was employed. cDNA Microarray analysis was performed by using a set of 5,184 sequence-verified rat cDNA clones. Results : Serum MMP and TIMP levels were not significantly different between the two groups. IL-6 was 36.82.8 pg/mL and TNF- 2.22.7 pg/mL in the ADR group. They were significantly higher than in the control group. Serum MMP correlated significantly with TNF-(r=0.41, P<0.05). There was no gene expression of MMP, IL-6 or TNF- in the hearts of both groups. Gene expression of TIMP was significantly depressed in the hearts of the ADR group. Conclusion : These results suggested a potential role for TNF- in the regulation of extracellular matrix remodeling in ADR induced cardiomyopathy. Rapid screening of multiple decreased gene expression by DNA chip may be a useful diagnostic test to detect early cardiac injury before developing ADR induced cardiomyopathy.
Keywords: Gene | Metalloproteinases | Cytokines | Adriamycin
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