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ORIGINAL ARTICLE
Korean J Pediatr 2005 August;48(8) :881-885.
Effects of Hyperosmolar Stimuli on Activation of Human Eosinophilic Leukaemia EoL-1 Cells
Byoung Chul Kwon (Kwon BC)1, Eun Soo Kim (Kim ES)1, Kyung Won Kim (Kim KW)1, Tae Won Song (Song TW)1, Myung Hyun Sohn (Sohn MH)2, Myeong Heon Shin (Shin MH)3, Kyu-Earn Kim (Kim KE)2
1Department of Pediatrics, BK 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea
2Department of Pediatrics, Institute of Allergy, BK 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea
3Department of Parasitology, Institute of Allergy, Institute of Tropical Medicine, BK 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea
Corresponding Author: Kyu-Earn Kim ,Email: kekim@yumc.yonsei.ac.kr
Copyright © 2005 by The Korean Pediatric Society
ABSTRACT
Purpose : Airway dehydration and subsequent hyperosmolarity of periciliary fluid are considered critical events in exercise-induced bronchoconstriction. The aim of this study was to establish if a hyperosmolar challenge could induce activation of eosinophils. Methods : Human eosinophilic leukaemic cell lines, EoL-1 cells were incubated with hyperosmolar solutions for 15 minutes. Activation of EoL-1 cells was monitored by degranulation and superoxide anion production. In addition, we examined surface expression of CD69 and ICAM-1. Results : Hyperosmolar stimuli didn't induce superoxide anion production and degranulation. In addition, EoL-1 cells cultured with hyperosmolar medium at 930 mOsm/kg H2O resulted in no significant increment in fluorescent intensity of CD69 and ICAM-1 expression compared with results for cells incubated with isomolar medium. Conclusion : We found that hyperosmolar stimuli don't cause activation of EoL-1 cells, but further studies are required to determine the role of eosinophil in the mechanism of exercise-induced asthma.
Keywords: Exercise-induced asthma | Hyperosmolar | EoL-1 cells
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