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Journal of the Korean Pediatric Society 1992;35(3):306-314.
Published online March 15, 1992.
The Effec of Conditiond Media Cultued from the Thymocytes and the Splenocytes on the Regulation of Antibody Isotypes; In Vitro Immunization-III
Dong Soo Kim, Geun Woong Noh, Soon Hwan Oh
Department of Pediatrics, Yonsei University College of Medicine, Seoul, Korea
비장세포와 흉성세포에서 유도한 조건화 배지의 항원 특이적 항체 Isotype의 조절에 관한 연구 ; 체외감작-III
김동수, 노건웅, 오순환
연세대학교 의과대학 소아과학교실
Abstract
We had developed a method of in-vitro immunization to increase antibody titer using several cnditioned media which were developed from mouse splenocyts and thymocytes. Using fetal calf serum, the splenocytes of the mouse were divided into two types; adherent cells and non-adherent cells. We used PHA to stimulate the adherent cells to produce PHA-stimulated adherent cell conditioned medium (ACSM-P). Non-adherent cells and thymocytes were stimulated by ACSM-P to produce ACSM-P-stimulaed non-adherent cell conditioned medium (NACSM-AP) and ACSM-P stimulaed thvmocvte conditioned medium (TmSM-AP) respectively. The highest antiody titer was observed among the methods of in-vitro immunizations. The effects of these conditioned media on producing antibody were tested in this repot. With the various combinations of these conditioned media, ivitro immunization was done, and antibody titier and isotypes of immunoglobulin were tested. ACSM-P in the presence of NACSM-AP had the effect to decrease IgM antiody response, but increase IgG reponse. On the other hands, ACSM-P in the absence of NACSM-AP showd to inhibit both IgM and IgG antibody response. TmSM-AP induced IgG antibody response, and also had anessential effect to induce IgM antibody response which was not observed in ACSM-P and NACSM-AP. All the conditioned media had their own roles to induce antibody response.l Further studies should be conducted to clartify the precise roles of these cnditioned media.
Key Words: In-Vitro Immunization
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