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Journal of the Korean Pediatric Society 1997;40(6):771-785.
Physical and Biological Activity of Domestic Product of Modified Bovine Lung Surfactant
Chul Lee1, Min Soo Park1, Moon Sung Park1, Jung Nyeon Kim1, Jong Wook Lee2, Kwang Hyun You2, Won Jae Kwag2, Kook In Park1, Ran Nam gung1, Dong Gwan Han1
1Department of Pediatrics, Yonsei University College of Medicine
2Yuhan Research Center
국내 개발된 Modified Bovine Lung Surfactant의 물리학적 및 생물학적 활성도
이철1, 박민수1, 박문성1, 김정녕1, 이종욱2, 유광현2, 곽원재2, 박국인1, 남궁란1, 한동관1
1연세대학교 의과대학 소아과학교실
2유한양행 중앙연구소
Abstract
Purpose
: Neonatal respiratory distress syndrome is caused by the deficiency of lung surfactant in premature babies. For the treatment of RDS at present surfactants such as Surfacten․ (Tokyo-Tanabe Co., Japan) and Exosurf․ (Wellcome Co., USA) are used. As awarded the grant from the Ministry of Science and Technology for a model research project of Medium-Technology program, we have modified (supplemented) the bovine lung extracts to get YY-38, for which we have performed physical and biological activities.
Methods
: For physical properties, we performed stable microbubble test (SMR) and measured surface tension lowering activity using a pulsating bubble surfactometer. Minimum and maximum surface tensions measured at 1 and 5 minutes gave surface tension-surface area diagrams, from which compressibility at surface tension 10mN/m was also calculated. As to the biological activity, we used premature rabbit fetuses as a model for the study of pressure-lung volume relationship. The lung pathology was examined on the lung tissues subsequently obtained, and aerated area ratios were calculated based on the area measured by an image analyzer.
Results
: The minimum surface tensions of YY-38 at 1 and 5 minutes for all different concentrations were low at 10mN/m, while the maximum surface tensions ranged from 33.01mN/m to 41.07mN/m. The surface tension-surface area curve showed a definite hysteresis at 1 and 5 minutes for all concentrations, and the surface tension fell below 10mN/m with 20% surface area compression. The compressibilities at surface tension 10mN/m at 5minutes for all concentrations were all below 0.02. In animal experiments, the mean lung volume of premature rabbit fetuses was inflated to 80.9ml/kg at maximum 30cmH2O, while the lung volume was maintained at 38.3mg/kg when the lung was deflated to 5cmH2O. The overall aerated area ratio was 45.4%.
Conclusion
: YY-38 formed sufficient amount of stable microbubbles and had a surface tension low enough to maintain alveolar stability and to exhibit a good hysteresis curve. In animal experiments it helped the expansion of premature lungs during inspiratory phase and was effective in the prevention of collapse during expiratory phase.
Key Words: Modified bovine lung surfactant, Fetal rabbit lung, Pressure-lung volume curve, Surface tension-surface area diagram
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