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Korean Journal of Pediatrics 2005;48(8):881-885.
Published online August 15, 2005.
Effects of Hyperosmolar Stimuli on Activation of Human Eosinophilic Leukaemia EoL-1 Cells
Byoung Chul Kwon1, Eun Soo Kim1, Kyung Won Kim1, Tae Won Song1, Myung Hyun Sohn2, Myeong Heon Shin3, Kyu-Earn Kim2
1Department of Pediatrics, BK 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea
2Department of Pediatrics, Institute of Allergy, BK 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea
3Department of Parasitology, Institute of Allergy, Institute of Tropical Medicine, BK 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea
고삼투압 자극이 호산구의 활성화에 미치는 영향
권병철1, 김은수1, 김경원1, 송태원1, 손명현2, 신명현3, 김규언2
1연세대학교 의과대학 소아과학교실, 두뇌한국21 의과학사업단
2연세대학교 의과대학 소아과학교실, 기생충학교실, 알레르기 연구소 , 두뇌한국21 의과학사업단
3기생충학교실, 알레르기 연구소, 열대의학 연구소, 두뇌한국21 의과학사업단
Correspondence:  Kyu-Earn Kim,
Email: kekim@yumc.yonsei.ac.kr
Abstract
Purpose
: Airway dehydration and subsequent hyperosmolarity of periciliary fluid are considered critical events in exercise-induced bronchoconstriction. The aim of this study was to establish if a hyperosmolar challenge could induce activation of eosinophils.
Methods
: Human eosinophilic leukaemic cell lines, EoL-1 cells were incubated with hyperosmolar solutions for 15 minutes. Activation of EoL-1 cells was monitored by degranulation and superoxide anion production. In addition, we examined surface expression of CD69 and ICAM-1.
Results
: Hyperosmolar stimuli didn't induce superoxide anion production and degranulation. In addition, EoL-1 cells cultured with hyperosmolar medium at 930 mOsm/kg H2O resulted in no significant increment in fluorescent intensity of CD69 and ICAM-1 expression compared with results for cells incubated with isomolar medium.
Conclusion
: We found that hyperosmolar stimuli don't cause activation of EoL-1 cells, but further studies are required to determine the role of eosinophil in the mechanism of exercise-induced asthma.
Key Words: Exercise-induced asthma , Hyperosmolar , EoL-1 cells
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